| Name | Time | Date | Meet |
|---|---|---|---|
| Tate Jackson | 41.06 | Dec. 1, 2018 | Texas Hall of Fame Invitational |
| Tate Jackson | 41.27 | Feb. 24, 2018 | Big XII Conference Championships |
| Jimmy Feigen | 41.49 | March 28, 2009 | 2009 NCAA Division I Mens Championships |
| Dave Walters | 41.92 | Dec. 6, 2008 | 2008 Texas Invitational |
| Garrett Weber-Gale | 41.94 | March 15, 2007 | 2007 NCAA Division I Mens Championships |
| Chris Kemp | 44.05 | March 6, 2003 | American Short Course Championships |
