| Name | Time | Date | Meet |
|---|---|---|---|
| Caspar Corbeau | 50.49 | March 25, 2022 | NCAA Division I Mens Championship |
| Will Licon | 50.68 | March 24, 2017 | 2017 NCAA Division I Mens Championships |
| Will Licon | 51.15 | Feb. 22, 2017 | Big XII Conference Time Trials |
| Scott Spann | 52.22 | March 27, 2010 | 2010 NCAA Division I Mens Championships |
| Tyler Ohalloran | 52.61 | March 15, 2007 | 2007 NCAA Division I Mens Championships |
| Christian Schurr | 54.81 | March 6, 2003 | American Short Course Championships |
