| Name | Time | Date | Meet |
|---|---|---|---|
| Kelsey Kisner | 28.11 | Feb. 10, 2023 | Old Dominion Athletic Conference Championships |
| Lizzy Ross | 28.42 | Nov. 23, 2019 | Randolph-Macon/Yellow Jacket Invitational |
| Lizzy Ross | 28.91 | Nov. 16, 2019 | Virginia Wesleyan vs. Salisbury |
| Lizzy Ross | 29.84 | Oct. 19, 2019 | Virginia Wesleyan vs. Randolph |
| Hayley Heath | 32.27 | Feb. 16, 2019 | Old Dominion Athletic Conference Championships |
| Hayley Heath | 32.70 | Dec. 8, 2018 | Salisbury vs. Virginia Wesleyan |
| Morgan Thomas | 48.32 | Oct. 20, 2018 | Virginia Wesleyan vs. Randolph |
